New Objective Posters & Presentations

Selected presentations and posters published at conferences and meetings can be found below.


Presentations at CPSA 2016

Covalent Bonds

Presented during the Vendor Session
"Five Minutes of Fame" - 5MOF


Presentations at ASMS 2016

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Increasing Operable Flow Range of a Nanospray Source for High-Performance Microspray on a Curtain Gas Equipped Triple–Quadrupole API-MS

Amanda Berg, Helena Svobodova, Gary Valaskovic
New Objective, Inc., Woburn, MA USA

Maximizing Flexibility: A Gas and Temperature Enabled Chip-Based Solution for Nanoflow and Microflow LC-MS

Aaron Dewberry, Helena Svobodova, Amanda Berg, Gary Valaskovic
New Objective, Inc., Woburn, MA USA


Presentations at CPSA 2015

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Picofuze: Integration Enables High-Flow Nanospray/Microspray With Conventional LC-ESI-MS

Gary Valaskovic1, Chad Christianson2, Jennifer Zimmer2, Shane Needham2
1 New Objective, Inc., Woburn, MA USA; 2 Alturas Analytics, Inc., Moscow, ID USA

Picochip: Development Of Chip-Based Nanobore Column Platform With Universal Connectivity, Column Heating, Sheath Gas Capability, And Multi-Plex Operation

Gary Valaskovic, Amanda Berg, Helena Svobodova,
New Objective, Inc., Woburn, MA USA


Presentations at ASMS 2015

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Evaluating and Improving the Stability of Nanospray for Operation at μL/min Flow Rates with an Integrated Chip-Based Solution

Helena Svobodova1, Amanda Berg1, Aaron Dewberry1, Peter Wang2, Gary Valaskovic1
1 New Objective, Inc., Woburn, MA USA; 2 New Objective, Shanghai, China

Improving NanoLC-MS Duty Cycle and Throughput: Three Channel Chip-based Column Switching Using a Linear Flow Path

Amanda Berg1, Helena Svobodova1, Aaron Dewberry1, Peter Wang2, Gary Valaskovic1
1 New Objective, Inc., Woburn, MA USA; 2 New Objective, Shanghai, China

PicoFuze: Integration Enables High-Flow Nanospray/Microspray with Conventional LC-ESI-MS

Corporate Poster - New Objective, Inc., Woburn, MA


Presentations at ASMS 2014

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Maximizing Nanoflow Spray Stability and Sensitivity using Automated Emitter Rinsing

Amanda Berg, Helena Svobodova, Gary Valaskovic
New Objective, Inc., Woburn, MA

Development of a Chip-Based Nanobore Column Platform with Universal Connectivity, Column Heating and Sheath Gas Capability

Helena Svobodova1, Peter Wang2, Amanda Berg1, Gary Valaskovic1
1 New Objective, Inc., Woburn, MA USA; 2 New Objective, Shanghai, China

The Integration of Nanoscale Separation and Ionization for the Analysis of Complex Proteomes

Tao Yi-Min1, Peter Wang2, Zhou Hu1, Yang Yi-Ming1, Helena Svobodova3, Amanda Berg3, Gary A. Valaskovic3
1 Shanghai Institute of Materia Medica, CAS, Shanghai, China; 2 New Objective, Inc., Shanghai, China; 3 New Objective, Inc., Woburn, MA

Development of a Robust, Accurate and Reproducible Procedure for Quantitative Analysis of Cardiac Troponin T using a Chip-Based Nanospray Source

Mariola Olkowicz1,2, Iwona Rybakowska3, Stefan Chlopicki4,5, Helena Svobodova6, Gary A. Valaskovic6, Ryszard Smolenski1
1 Department of Biochemistry, Medical University of Gdansk; 2 Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Poland; 3 Department of Biochemistry and Clinical Physiology, Medical University of Gdansk, Poland; 4 Department of Experimental Pharmacology, Jagiellonian University Medical College, Poland; 5 Jagiellonian Centre for Experimental Therapeutics, Jagiellonian University, Poland; 6 New Objective Inc., USA

Finding the Appropriate LC Setup for Proteomics Experiments Depending on the Sample Complexity using Chip-Based Columns

Jan Muntel1,2;Helena Svobodova3; Gary A. Valaskovic3; Saima Ahmed1,2; Kevin Broadbelt1,2; Omar Barnaby1,2; Hanno Steen1,2
1 Proteomics Center at Boston-Children’s Hospital, Boston, MA; 2 Department of Pathology Boston Children’s Hospital and Harvard Medical School, Boston, MA; 3 New ObjecHve, Inc., Woburn, MA

Presentations at CPSA 2013

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Performance Evaluation of a Flexible, Easy-to-Use Packed-Tip Column Device for Nanospray Enabled LC-MS

Helena Svobodova, Amanda Berg, Ben Ngo, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Evaluating Nanospray Ruggedness on a Curtain Gas-Triple Quadrupole MS Equipped with Emitter Rinsing

Amanda Berg, Helena Svobodova, Ben Ngo, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Breaking the NanoLC-MS Throughput Barrier: High-Performance at 95% Duty-Cycle

Helena Svobodova, Amanda Berg, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Presentations at ASMS 2013

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Performance Evaluation of a Flexible, Easy-to-Use Packed-Tip Column Device for Nanospray Enabled LC-MS

Helena Svobodova, Amanda Berg, Ben Ngo, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Evaluating the Ruggedness of Nanospray on a Curtain Gas-Triple Quadrupole MS Equipped with Emitter Rinsing

Amanda Berg, Helena Svobodova, Ben Ngo, Gary A. Valaskovic
NewObjective Inc, Woburn, MA

A Digitized Workflow for Static Nanospray Mass Spectrometry: Combining High Throughput, Efficiency and Ease of Use

Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Breaking the NanoLC-MS Throughput Barrier: High-Performance at 95% Duty-Cycle

Benjamin Ngo, Amanda Berg, Carla Marshall-Waggett, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Presentations at HUPO 2012

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Increased Sample Loading Capacity For Peptide Analysis by LC-MS/MS Using 150 µm ID Packed-Tip Columns

Helena Svobodova, Amanda Berg, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Ruggedness of Nanobore LCMS for Qualitative and Quantitative Biomarker Analysis Using an Automated Emitter Positioning and Rinsing System

Amanda Berg, Carla Marshall-Waggett, Gary Valaskovic
NewObjective Inc, Woburn, MA

The PicoChip: A Robust, Easy-to-Use Solution for Nanospray Enabled LC-MS

Benjamin Ngo, Amanda Berg, Carla Marshall-Waggett, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Presentations at ASMS 2012 -Vancouver, BC

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Robust Dual-Column Source for Improving Nano-LCMS Duty Cycle

Benjamin Ngo, Helena Svobodova, Amanda Berg, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Characterization of the Key Tuning Parameters in NanoESI Analysis using an Automated Positioning System

Amanda Berg1, Mike S. Lee2, Gary A. Valaskovic1
1New Objective, Inc., Woburn, MA; 2Milestone Development Services, Newtown, PA

Improving Quantitative nanospray LC-MSMS Workflows through Voltage Control

Gary A. Valaskovic1, Amanda Berg1, Mike S. Lee2
1New Objective, Inc., Woburn, MA; 2Milestone Development Services, Newtown, PA

The PicoChip: A Robust, Easy-to-Use Solution for Nanospray Enabled LC-MS

Benjamin Ngo, Amanda Berg, Carla Marshall-Waggett, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Presentations at CPSA 2011 - Langhorne, PA

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Evaluation of 150µm ID Packed-Tip Columns for Qunatitative Peptide Analysis by LC-MS/MS

Stanley Durand, Helena Svobodova, Amanda Berg, Gary A. Valaskovic
1New Objective, Inc., Woburn, MA; 2Milestone Development Services, Newtown, PA

Optimization of Nanospray Voltage and Spray Stability: Impact on Chromatographic Peak Area for Analyte Quantitation

Amanda Berg1, Mike S. Lee2, Gary A. Valaskovic1
1New Objective, Inc., Woburn, MA; 2Milestone Development Services, Newtown, PA

Fundamental Characteristics of Small Molecule Analysis Using Ultra-Low Dynamic Flow

Ben Ngo1; Shaoxia Yu2; Mike S. Lee3; Jing-Tao Wu2; Gary Valaskovic1
1New Objective, Inc., Woburn, MA; 2Millennium Pharmaceuticals, Inc., Cambridge, MA; 3Milestone Development Services, Newtown, PA

Presentations at ASMS 2011 - Denver, CO

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Improving Nanobore Column Duty Cycle via Trap-Column Injection: Evaluating the Effect of Trap-Column Injection Flow Rate on Analytical Separation

Carla Marshall-Waggett, Helena Svobodova, Amanda Berg, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Evaluation of 150µm ID Packed-Tip Columns for Qunatitative Peptide Analysis by LC-MS/MS

Stanley Durand, Helena Svobodova, Amanda Berg, Gary A. Valaskovic
1New Objective, Inc., Woburn, MA; 2Milestone Development Services, Newtown, PA

Optimization of Nanospray Voltage and Spray Stability:Impact on Chromatographic Peak Area for Analyte Quantitation

Amanda Berg1, Mike S. Lee2, Gary A. Valaskovic1
1New Objective, Inc., Woburn, MA; 2Milestone Development Services, Newtown, PA

Fundamental Characteristics of Small Molecule Analysis Using Ultra-Low Dynamic Flow

Ben Ngo1; Shaoxia Yu2; Mike S. Lee3; Jing-Tao Wu2; Gary Valaskovic1
1New Objective, Inc., Woburn, MA; 2Millennium Pharmaceuticals, Inc., Cambridge, MA; 3Milestone Development Services, Newtown, PA

Presentations at ABRF 2011 - Sacramento, CA

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Increased Sample Loading Capacity For Peptide Analysis by LC-MS/MS Using 150 µm ID Packed-Tip Columns

Amanda Berg, Helena Svobodova, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Evaluation of Nanospray Voltage and Spray Stability and their Impact on Chromatographic Peak Area

Gary A. Valaskovic1, Mike S. Lee2, Amanda Berg1
1New Objective, Inc., Woburn, MA; 2Milestone Development Services, Newtown, PA

Flow Rate, Concentration and Saturation: Investigating the Fundamentals of Sample-Trapped Column Injection

Carla Marshall-Waggett, Amanda Berg, Helena Svobodova, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Presentations at CPSA 2010 - Langhorne, PA

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Optimization of Sample Loading Capacity for Absolute Peptide Quantification by Nanobore LC-MS/MS

Amanda Berg1, Gary A. Valaskovic1, Chuck Witkowski2, Jeremy L. Norris2, Mike S. Lee3
1New Objective, Woburn, MA; 2Protein Discovery, Knoxville, TN; 3Milestone Development Services, Newtown, PA

Nanobore RPHPLC: Determining The Role of Selectivity in Method Development

Amanda Berg, Carla Marshall-Waggett, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

On-line Microfluidic Extraction Enables Highly Efficient and Sensitive Direct Elution from Dried Blood Spots

Gary A. Valaskovic1; Christopher A. Evans2; Chester L. Bowen2
1New Objective, Inc., Woburn, MA; 2GlaxoSmithKline, King Of Prussia, PA

Flow Rate, Concentration and Saturation: Investigating the Fundamentals of Sample-Trapped Column Injection

Carla Marshall-Waggett, Amanda Berg, Helena Svobodova, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Presentations at ASMS 2010 - Salt Lake City, UT

(Files may require Adobe Acrobat Reader or MS Powerpoint)

On-line Microfluidic Extraction Enables Highly Efficient and Sensitive Direct Elution from Dried Blood Spots

Gary A. Valaskovic1; Christopher A. Evans2; Chester L. Bowen2
1New Objective, Inc., Woburn, MA; 2GlaxoSmithKline, King Of Prussia, PA

Advances in Capillary Liquid Chromatography for High-Throughput Top Down Proteomics

Adam D. Catherman1, John C. Tran1, Lee Sawdey3, Kenneth R. Durbin1, Adaikkalam Vellaichamy2, Gary Valaskovic3, Neil L. Kelleher1,2
1Department of Chemistry, 2The Institute for Genomic Biology, University of Illinois, 3New Objective, Inc

Bridging the Gap Between Nanospray and ESI with Capillary Spray

Amanda Berg, Carla Marshall-Waggett, Gary Valaskovic
New Objective, Inc., Woburn, MA

Optimization of Sample Loading Capacity for Absolute Peptide Quantification by Nanobore LC-MS/MS

Amanda Berg1, Gary A. Valaskovic1, Chuck Witkowski2, Jeremy L. Norris2, Mike S. Lee3
1New Objective, Woburn, MA; 2Protein Discovery, Knoxville, TN; 3Milestone Development Services, Newtown, PA

Presentations at ABRF 2010 - Denver, CO

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Nanobore RPHPLC: Determining The Role of Selectivity in Method Development

Amanda Berg, Carla Marshall-Waggett, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Presentations at CPSA 2009 - Langhorne, PA

Novel and Robust ESI Source for Efficient Bioanalytical Workflows at Capillary LC Flow Rates

Amanda Berg, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

From Sample to Spray: High Performance Workflow for Top-Down Protein Analysis

1Chuck Witkowski, 1Jeremy Norris, 2Gary Valaskovic, 3Top-Down Development Team, 3Neil Kelleher
1Protein Discovery, Inc., Knoxville, TN; 2New Objective, Inc., Woburn, MA; 3Center for Top-Down Proteomics, University of Illinois, Champagne-Urbana

Presentations at ASMS 2009 - Philadelphia, PA

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Towards Robust Quantitative MRM Plasma Analysis using Nanobore Liquid Chromatogrpahy Through Improves Nanoelectrospray Performance

Susan E. Abbatiello1, Amanda Berg2, Gary Valaskovic2, Steven A. Carr 1
1The Broad Institute, Cambridge, MA; 1NewObjective, Woburn, MA

Minimization of Atmospheric Background Contaminants in Nanoelectrospray: Identification and Optimization

Benjamin Ngo, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Fundamental Studies on the Electrophoretic Enrichment of Peptides in Clinical Samples for Quantification using nanoLC-MS/MS

Jeremy L. Norris1, Gary Valaskovic2, Mike S. Lee3, Chuck Witkowski1
1Protein Discovery, Inc., Knoxville, TN; 2New ObjectiveInc, Woburn, MA; 3Milestone Development Services, Newtown, PA
Not available for download

Top Down Proteomics Using Online Polymer Reversed Phase (PLRP) Nanocapillary-LC-Coupled Fourier Transform Mass Spectrometry

Adaikkalam Vellaichamy1, John Kellie1, John C. Tran1, Ji Eun Lee1Jaeyun Sung1,
Nathan D. Price1, Gary Valaskovic2, Neil L. Kelleher1
1University of Illinois, Urbana, IL; 2New Objective Inc, Woburn, MA

Comparison of Chromatographic Media for Nano ESI LC-MS/MS: Applications to Biomarker Discovery

Michael Gardner1, Megan Rowland2, Jonathan L.Bundy1, James Stephenson1, Gary Valaskovic3, Mike S. Lee4
1Research Triangle Institute, RTP, NC; 2RTI International, Cary, NC; 3New Objective Inc, Woburn, MA; 4Milestone Development Services, Newtown, PA
Not available for download

Ruggedness of Nanobore LCMS for Qualitative and Quantitative Biomarker Analysis using an Automated Emitter Positioning and Rinsing System

Amanda Berg, Carla Marshall-Waggett, Gary Valaskovic
NewObjective Inc, Woburn, MA

Improved Throughput for Clinical Proteomics Using a Dual Channel NanoLC-Nanospray Source

Cheryl F. Lichti1, Benjamin Ngo2, Marjorie N. Case1, R.Reid Townsend1, Gary A. Valaskovic2
1Washington University School of Medicine, St. Louis, MO; 2New Objective Inc, Woburn, MA

Comparison of Conventional, Sub-2-µm, and Superficially Porous (Fused-Core) Particles for Long and Short Gradient Nanobore LC/MS

Robert Moody1, Gary A. Valaskovic2, Mike S. Lee3
1MAC-MOD Analytical, Chadds Ford, PA; 2New Objective Inc, Woburn, MA; 3Milestone Development Services, Newtown, PA

Temperature Control Effects in Packed Emitter Nanobore LC-MS/MS for Protein Digest Analysis

Mike S. Lee1, Lee Swadey2, Amanda Berg2, Gary A. Valaskovic2
1Milestone Development Services, Newtown, PA; 2NewObjective Inc, Woburn, MA

Spatial Mapping of Analyte Ion Intensity of Nanoelectrospray in the Plume-Inlet Region

Gary A. Valaskovic1, Mike S. Lee2
1New Objective Inc, Woburn, MA; 2Milestone Development Services, Newtown, PA

High Sensitivity Nanoscale Multidimensional LC/MS Analysis of Phosphopeptides

Scott Ficarro1, Yi Zhang2, Feng Zhou1,Bob Cardoza1, Guillame Adelmont1, Manor Askenazi2, Amanda Berg3, Gary A. Valaskovic3, Jared Marto1
1Dana-Farber Cancer Institute, Boston, MA; 2Dana-Farber Cancer Institute and Hebrew University, Boston, MA; 3New Objective Inc, Woburn, MA
Not available for download

Posters Presented at ABRF 2009 - Memphis, Tennessee

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Imaging of Nanoelectrospray Spray Current with an Automated Digital Control Positioning System

Gary A. Valaskovic1, Mike S. Lee2
1New Objective, Woburn, MA; 2Milestone Development Services,Newtown, PA

Nanoelectrospray Ruggedness for Qualitative and Quantitative Biomarker Analysis Using an Automated Emitter Positioning and Rinsing System

Amanda Berg, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Minimization of Atmospheric Background Contaminants in Off- and Online Nanoelectrospray: Identification and Optimization

Benjamin Ngo, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Posters Presented at ASMS 2008 - Denver, Colorado

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Optimization of Nanoelectrospray with Plasma DerivedSamples for Qualitative and Quantitative Biomarker Analysis

Jeffrey Wynn, Amanda Berg, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Performance Comparison of Standard Electrospray vs. Nanospray for the Quantification of Nucleopeptides and their Phosphorolated Metabolites

John D. Lennon1, Ken Lewis1, Gary A Valaskovic2
1OpAns, LLC, Durham, NC; 2New Objective Inc, Woburn, MA
Not available for download

Optimization of Novel Fused-Core Silica Particle Nanobore Columns Using a Pump-Switching Motif for Mass Spectrometry

Robert Moody1; Gary A. Valaskovic2; Mike S. Lee3
1MAC-MOD Analytical, Chadds Ford, Pennsylvania; 2New Objective, Inc., Woburn, MA; 3Milestone Development Services, Newtown, PA

High-Sensitivity Nanocapillary Chromatography Combined With Active Background Ion Reduction Increases Signal-to-Noise Ratios, Sensitivity,and Overall System Performance

John M. Neveu1, Bogdan A. Budnik1, GaryA. Valaskovic2, William S. Lane1
1Harvard University, Cambridge, MA;2New Objective, Inc., Woburn, MA

Maximizing Performance of sub-2 µm Packed Nanobore LC Columns: Minizing Extra-Column Variance

Carla J . Marshall-Waggett, Amanda L. Berg, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Investigation of Elastomer Core Compression Effect on Minimizing Trapping Column Band Broadening

Amanda L. Berg, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Measurement of Testosterone in Human Urine by Direct Injection Using nano-Pump Switching with Nanospray Tandem Mass Spectrometry

Daniel Magiera1, Mike S. Lee2, Gary A. Valaskovic3
1Molecular MS Diagnostics, LLC, Cranston, RI; 2MilestoneDevelopment Services, Newtown, PA; 3New Objective, Inc., Woburn, MA
Not available for download

Imaging of Nanoelectrospray Spray Current with an Automated Digital Control Positioning System

Gary A. Valaskovic1, Mike S. Lee2
1New Objective, Woburn, MA; 2Milestone Development Services,Newtown, PA

Improving the Sensitivity of LC/MS Assays for Low-Abundance Protein Biomarkers: Bridging the Gap with ELISA

Lee W. Ott1, Michael J. Berna1, Gary A. Valaskovic2, Bradley L. Ackermann1
1Eli Lilly & Co., Greenfield, IN; 2New Objective, Inc., Woburn, MA
Not available for download

Posters Presented at ASMS 2007 - Indianapolis, Indiana

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Correlating Extended Dynamic Range with Nanospray Flow Rate Measurement for Optimal ESI-MS Performance

Carla J. Marshall-Waggett, Christopher J. Toher, Gary A. Valaskovic
New Objective, Woburn, MA

Inline Electrospray Current Conductivity Detection for Characterization of Mobile Phase Composition and Gradient Delay

Adam W. Perala, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Efficient Column-to-Column Coupling with Multiple Columns for Enhanced nLC-ESI-MS Separation

Christopher Toher, Adam W. Perala, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

A Novel Fused-core Silica Particle Based Nanobore Column for Ultra-high Performance Nanoelectrospray Mass Spectrometry

Robert Moody1; Gary A. Valaskovic2; Mike S. Lee3
1MAC-MOD Analytical, Chadds Ford, Pennsylvania, 2New Objective, Inc., Woburn, MA, 3Milestone Development Services, Newtown, PA
Not available for download

Ultra-Performance Peptide Analysis via Nanobore LC-ESI-MS with Sub-2µm Particles

John M. Neveu1, William S. Lane1, Gary A. Valaskovic21Harvard University, Cambridge, MA;
2New Objective, Inc., Woburn, MA

Digitized Nanobore LC-MS: An Automated Control System with Emitter Divert for Column-Switching

Mike S. Leesup>1, Gary A. Valaskovic21Milestone Development Services, Newtown, PA, 2New Objective, Inc., Woburn, MA

Evaluation of Fluoropolymer Frit Performance in High-pH Nano-ESI Applications

Jeffrey Wynn, Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective Inc, Woburn, MA

On-line 1D and 2D nanoLC-ESI-MS using 10-µm-i.d. Porous Layer Open Tubular Polystyrene-divinylbenzene Columns for Ultrasensitive Proteomic Analysis

Quanzhou Luo1, Guihua Yue1, Gary A. Valaskovic2, Ye Gu1, Dongdong Wang1, Shiaw-Lin Wu1, Barry L. Karger1
1The Barnett Institute, Northeastern University, Boston, MA, 2New Objective, Inc., Woburn, MA
Not available for download

Posters Presented at ABRF 2007 - Tampa, Florida

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Inline Electrospray Current Conductivity Detection for Characterizing Mobile Phase Composition and Gradient Delay

Gary A. Valaskovic, Adam W. Perala, Christopher J. Toher
New Objective Inc, Woburn, MA

Long- & Mixed-Column Nanobore Chromatography for Complex Proteomic Analysis

Christopher J. Toher, Adam W. Perala, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Examination of Nanobore Capillary Trapping Column Geometries for Analysis of Peptide Mixtures

John M. Neveu1, Christopher J. Toher2, William S. Lane1, Gary A. Valaskovic2
1Harvard University, Cambridge, MA   2New Objective, Woburn, MA

Rapid Nanobore LC-ESI-MS Peptide Analysis Using Sub-3 µm-Diameter Resins

Adam W. Perala, Christopher J. Toher, Carla J. Marshall-Waggett, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Posters Presented at ASMS 2006 - Seattle, Washington

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Optimization of Nanobore Capillary Trapping Column Geometries for Analysis of Peptide Mixtures

John M. Neveu1, Christopher J. Toher2, William S. Lane1, Gary A. Valaskovic2
1Harvard University, Cambridge, MA   2New Objective, Woburn, MA

High-Pressure, Transparent Microfluidic Coupling Method for Nanobore LC-MS

Jeffrey J. Wynn, Christopher J. Toher, Adam W. Perala, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Digitized Nanobore LC-MS Control System with Integrated Emitter Divert & Rinse for Improved Nanospray Performance

James L. Stephenson1, Jonathan L. Bundy1, Gary A. Valaskovic2, Mike S. Lee3
1RTI International, Research Triangle Park, NC   2New Objective Inc, Woburn, MA   3Milestone Development Service, Inc., Newtown, PA

Offline Nano-ESI Phosphopeptide Analysis with TiO2, and ZrO2 Wall-Coated Trap'nTips™

Christopher J. Toher1, Adam W. Perala1, Ashok K. Shukla2, Gary A. Valaskovic1
1New Objective Inc, Woburn, MA, 2Glygen,Inc., Columbia, MD

Posters Presented at ABRF 2006 - Long Beach, California

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Offline Nano-ESI Phosphopeptide Analysis with Carbon, TiO2, and ZrO2
Wall-Coated Trap'nTips™

Christopher J. Toher1, Adam W. Perala1, Ashok K. Shukla2, Gary A. Valaskovic1
1New Objective Inc, Woburn, MA, 2Glygen, Inc., Columbia, MD

PicoClear™ Union Technology Applications for Nano ESI-MS Molecules

Carla J. Marshall-Waggett, Adam W. Perala, Christopher J. Toher,Gary A. Valaskovic
New Objective Inc, Woburn, MA

Evaluation of Fluoropolymer Frit Performance in High-pH Nano-ESI Applications

Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Posters Presented at ASMS 2005 - San Antonio, Texas

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Automated Orthogonal ESI Control System with Optimized Plume-Inlet Positioning

Mike S. Lee 1, Gary A. Valaskovic2
1Milestone Development Services, Inc., 2New Objective, Inc., Woburn, MA

Sample Purification for Static Nanospray MS Using Wall-Coated Pipette Trap'nTips™

Christopher J. Toher 1, Adam W. Perala 1, Ashok K. Shukla 2, Gary A. Valaskovic1
1New Objective Inc, Woburn, MA, 2Glygen, Inc., Columbia, MD

Enhanced Nanobore LC-MS Using Methanol Gradient Elution with Peptide Mixtures

Adam W. Perala, Christopher J. Toher, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

TIC-Mediated Flow Rate Variation for Flow-Injection NanosprayAnalysis

Carla J. Waggett1, David Neyer2, Adam W. Perala1, Christopher J. Toher1, Gary A. Valaskovic1
2New Objective, Inc., Woburn, MA, 2Eksigent Technologies, Livermore, CA

Charged Droplet Solvent Extraction from Surfaces: Desoprtion Electrospray Ionization of Small Drug Molecules

Gary A. Valaskovic1, Mike S. Lee2
1New Objective Inc, Woburn, MA, 2Milestone Development Services, Inc.

Posters presented at ABRF 2005 - Savannah, GA

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Automated ESI Control for Variable Flow Nanobore LC-MS

Gary A. Valaskovic1, Mike S. Lee2
1New Objective Inc, Woburn, MA, 2Milestone Development Services, Newtown, PA

Posters Presented at ASMS 2004 - Nashville, TN

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Method to Optimize Sensitivity and Throughput on a Hybrid Quadrupole-TOF Instrument for LC/MS-Based Peptide and Proteomic Analysis

Joseph Bonapace1, Jennifer Rutherford1, James P. Murphy III2, Gary A. Valaskovic2
1Charles River Proteomic Services, Worcester, MA 2New Objective Inc, Woburn, MA

Automated Orthogonal Control System for Electrospray Ionization Mass Spectrometry

Gary A. Valaskovic1, James P. Murphy III1, Mike S. Lee2
1New Objective Inc, Woburn, MA, 2Milestone Development Services, Newtown, PA

Minimizing Post-Column Band Broadening in Nanobore LC-MS/MS for Bottom-up Proteomic Applications

Andreas Huhmer1, James P. Murphy III2, Gary A. Valaskovic2
1Thermo Electron Corp., San Jose, CA, 2New Objective, Inc., Woburn, MA

Fundamental Studies on the Application of Nanobore LC-MS for the Analysis of Small Drug Molecules

Mike S. Lee1, James P. Murphy III2, Gary A. Valaskovic2
1Milestone Development Services, Newtown, PA, 2New Objective, Inc., Woburn, MA

Automated ESI Control on Variable-Flow Gradient Nanobore LC-MS

James P. Murphy III1, Gary A. Valaskovic1, Karen M. Hahnenberger2, David W. Neyer2
1New Objective Inc, Woburn, MA, 2Eksigent Technologies, Livermore, CA

Posters Presented at ABRF 2004 - Portland, OR

(Files may require Adobe Acrobat Reader or MS Powerpoint)

An Automated Control System for Nano-Electrospray

James P. Murphy III, Gary A. Valaskovic
New Objective Inc, Woburn, MA

Posters Presented at ASMS 2003 - Montreal, Quebec, Canada

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Fabrication and Use of 20 µm ID Nanobore Columns

Gary A. Valaskovic and James P. Murphy III
New Objective, Inc., Woburn, MA
Not available for download

Trap/Column Combination to Optimize Sensitivity and Sequence Coverage

James P. Murphy III and Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Variable Flow Gradient LC-MS: Spray Mode Signal Affects and Control

Mike S. Lee, Gary A. Valaskovic, and James P. Murphy III
New Objective, Inc., Woburn, MA

Poster Presented at ABRF 2003

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A Comprehensive Study on Nanobore Injection Methods for Proteomics

James P. Murphy III, Gary A. Valaskovic
New Objective, Inc., Woburn, MA

Industrial Seminar Presented at HPCE 2003

(Requires MS Powerpoint)

Technical Abstracts from ASMS 2002

(Files may require Adobe Acrobat Reader or MS Powerpoint)

Improved Nanospray Emitter Coatings for Nanospray LC-MS (Abstract only)

James P. Murphy III and Gary A. Valaskovic
New Objective, Inc, Woburn, MA

Taylor Cone Stability and ESI Performance for LC-MS at Low Flow Rates (Abstract only)

1Mike S. Lee, 2James P. Murphy III, 2Gary A. Valaskovic
1Milestone Development Services, Newtown, PA 2New Objective Inc, Woburn, MA

Orthogonal Control Systems for ESI-MS and Nanobore LC-MS (Abstract only)

1Gary A. Valaskovic, 1James P. Murphy III, 2Mike S. Lee
1New Objective Inc, Woburn, MA 2Milestone Development Services, Newtown, PA

Poster Presented at Systeomics 2002

Comparison of Nanobore LC/MS Injection Methods for Proteomics

James P. Murphy III and Gary A. Valaskovic
New Objective, Inc., Woburn