Leaders in Flow Technology™
"Our facility's major proteomic application is to produce the most comprehensive map of proteins from rat liver organelles. This "Cell Map" project requires the use of a nanoflow column that is robust, reliable and works on a 24/7 basis. To this end, in April of 2001 we selected to use PicoFrit columns from New Objective. One of our columns has been running for more than 170 days now. Over 1,700 samples have been analyzed without losing chromatographic resolution. The long column life and chromatographic reproducibility between each run has assured this technology will remain an integral component of the RPMPN proteomics pipeline for years to come."
- Daniel Boismenu, Ph.D.
Montreal Proteomics Network
IntegraFrit™ columns are the building blocks of your high performance analytical method. The exceptional flexibility, consistency and stability are the hallmark of these non-metal fritted columns, and allow the full potential of nanospray to be harnessed with the benefits of superior control and performance independent of your analysis platform.
Like PicoFrit® columns, IntegraFrit columns are fabricated from 360 µm OD, polyimide-coated, fused-silica tubing, but without a tip. The fritted end of the fused-silica column is polished flat to ensure a clean connection to your emitter of choice or to the inlet of a second column. Each IntegraFrit column has an integral high-porosity frit, behind which is the packed chromatography bed. The frit virtually eliminates peak tailing caused by other extra-column frits.
Coupling columns of the same or different resin materials is often employed in complex proteomic digest analysis. Despite enhanced separation, multidimensional columns are costly, and initiate post-column through dead-volume introduction. Using transparent, true zero-dead-volume PicoClear™ unions in conjunction with IntegraFrit columns, flush connections can easily be achieved, and rapid swap-out of column materials is made possible. Data collected using bench-top assembled columns demonstrate negligible resolution loss.
IntegraFrit columns are exceptional for biphasic and multi-dimensional analysis. Combining your preferred chemistry with SCX media in a single bed eliminates excesses of both swept- and dead- volumes.
Multi-phasic columns can also be produced according to customer specification. Contact New Objective for more information and pricing.
Like PicoFrit columns, IntegraFrit columns are available without media, fritted and ready to be packed in your lab. But IntegraFrits can be used in a number of ways...
CREATE LONG- & MIXED-BED COLUMNS
Contact New Objective for pricing.
Academic institutions receive a 5% discount on PicoChip columns. Academic discount cannot be combined with other discounts or promotions.
PicoFrit, PicoChip, and IntegraFrit columns are available packed with any of our available premier chemistries. Please contact New Objective for more information on any configuration not listed here.
PicoFrit/PicoChip columns are manufactured under U.S. Patents 5,997,746 and 6,190,559 and are sold for use under license of U.S. Patent 5,572,023. Other patents pending.