What is the difference between SilicaTips™ and TaperTips™?
SilicaTip™ and TaperTip™ emitters are made from the highest-quality fused silica. The main difference between them is the shape of the tip. SilicaTip emitters have tapered ends, meaning that the tip opening is smaller than the actual ID of the tubing. TaperTip emitters do not have an internal taper; they only have an external taper, and the ID of the tip opening is the same as the ID of the tubing.
In general, SilicaTip emitters provide a more stable spray, especially at nano flow rates below 500 µL/min, while TaperTip emitters are more robust and can also be used for higher flow applications (>1 µL/min).
What differentiates New Objective emitters from other emitters in the market?
With decades of experience, New Objective provides high-performance emitters designed for nanoflow rates. Our products are engineered to be robust, reproducible, and reliable. Each emitter undergoes a stringent manufacturing process and individual inspection via automated video microscopy to guarantee quality. New Objective SilicaTips feature molecular-level sharpness with an OD that virtually matches the ID, providing exceptional spray stability. With ID and OD tolerances maintained at the micrometer level, our strict QC criteria ensure consistent results from emitter to emitter and box to box.
What application are the emitters used for?
The GlassTips™ emitters work well for native protein analysis and static nanospray applications. The SilicaTips and TaperTips can be used for higher flow rates compared to the GlassTips. SilicaTips can be used for online nanospray and TaperTips can be used for nano to microspray.
What is the average lifetime of the emitters?
The average lifetime of the emitters are highly sample dependent. We have had customers report operational lifetimes exceeding many months when operated in optimal circumstances.
Are GlassTips available with coating?
GlassTips are available as coated emitters and noncoated emitters. Voltage can be applied directly on the coated region of the coated GlassTip emitters. A platinum wire can be used to apply voltage to noncoated emitters.
Can the emitters be coupled with a column?
New Objective SilicaTip and TaperTip can be coupled to a column. There are many labs that are using this setup.
What instrumentation are the emitters compatible with?
The New Objective emitters are compatible with most mass spectrometers and LC systems. Consult your MS vendor or reach out to sales@newobjective.com for more information.
What is the preferred flow rate for each type of emitter?
The typical flow rates for SilicaTips are less than 1 uL/min and for TaperTips the typical flow rates 1 uL/min to 10 uL/min.
The typical flow rates for GlassTip emitters range from 5 – 100 nL/min. The flow rate is determined by the orifice size, applied voltage, and back pressure.
Are New Objective emitters robust?
SilicaTips and TaperTips are both very robust options for LC-MS applications. The tip shape of the TaperTip emitters makes these emitters less likely to clog in case particles are introduced into the emitter.
Are the GlassTip emitters reusable?
GlassTips are a single use consumable designed to be used for a single sample and single analysis.